Plasmid

Part:BBa_K3904319:Design

Designed by: Bernadeta Aleksandravičiūtė   Group: iGEM21_Vilnius-Lithuania   (2021-09-30)


pET-28a(+) with N-His tag and TEV recoginition sequence


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5161
    Illegal XbaI site found at 5030
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5161
    Illegal NheI site found at 5122
    Illegal NotI site found at 5186
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5161
    Illegal BglII site found at 4964
    Illegal BamHI site found at 5155
    Illegal XhoI site found at 5195
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5161
    Illegal XbaI site found at 5030
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5161
    Illegal XbaI site found at 5030
    Illegal NgoMIV site found at 137
    Illegal NgoMIV site found at 3184
    Illegal NgoMIV site found at 3344
    Illegal NgoMIV site found at 4932
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2263


Design Notes

.


Source

Synthetic.

References